Coding

Part:BBa_K3552001

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)
Revision as of 10:16, 25 October 2020 by Registry (Talk | contribs)

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GmPilA

GmPilA is a type 4 pilus generally on Geobacter metallireducens which can conduct electricity. This part is in the part collection where we provide different conductive pilus based on the same pilin generator. By these different type 4 pilus we can compare the qualities.

GmPilA are fimbrial protein which normally grow on Geobacter metallireducens. They are type 4 pili which are long, and thin that displayed on the cell surface membrane. They can promote adherence, motility and transport functions in the bacteria. They are mainly built as helical polymers of a single subunit called the major pilin. These pilins are initially in the plasma membrane and the N-terminal is positively charged. The mature pilins will be extracted through membrane by the pilin generator. The G. Metallireducens pili at pH 7 has a higher conductivity than the G. Sulfurreducens pili under similar preparation and this is because a phenylalanine and tyrosine in GsPilA were replaced by a tryptophan.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None