Part:BBa_K3606065:Design
P9 driven mcbABCD and PtetR driven mcbEFG
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5547
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal NotI site found at 5539 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5547
Illegal BamHI site found at 3366 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal NgoMIV site found at 2032
Illegal AgeI site found at 2204 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.
Source
McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.
References
Collin F, Maxwell A. The Microbial Toxin Microcin B17: Prospects for the Development of New Antibacterial Agents. J Mol Biol. 2019;431(18):3400–3426. doi:10.1016/j.jmb.2019.05.050
S. Duquesne, D. Destoumieux-Garzón, J. Peduzzi, S. Rebuffat. Microcins, gene-encoded antibacterial peptides from enterobacteria
Sassone-Corsi M, Nuccio SP, Liu H, Hernandez D, Vu CT, Takahashi AA, Edwards RA, Raffatellu M. Microcins mediate competition among Enterobacteriaceae in the inflamed gut. Nature. 2016 Dec 8;540(7632):280-283.
Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PMID: 23474465.