Part:BBa_K3606069:Design

P13 driven mcbABCD and PtetR driven mcbEFG

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4794
  Illegal PstI site found at 2297
  Illegal PstI site found at 2330
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4794
  Illegal PstI site found at 2297
  Illegal PstI site found at 2330
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4794
  Illegal BglII site found at 2204
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4794
  Illegal PstI site found at 2297
  Illegal PstI site found at 2330
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4794
  Illegal PstI site found at 2297
  Illegal PstI site found at 2330
  Illegal NgoMIV site found at 1959
  Illegal AgeI site found at 2131
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5055
  

Design Notes

McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.

Source

McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.

References

Collin F, Maxwell A. The Microbial Toxin Microcin B17: Prospects for the Development of New Antibacterial Agents. J Mol Biol. 2019;431(18):3400–3426. doi:10.1016/j.jmb.2019.05.050

S. Duquesne, D. Destoumieux-Garzón, J. Peduzzi, S. Rebuffat. Microcins, gene-encoded antibacterial peptides from enterobacteria

Sassone-Corsi M, Nuccio SP, Liu H, Hernandez D, Vu CT, Takahashi AA, Edwards RA, Raffatellu M. Microcins mediate competition among Enterobacteriaceae in the inflamed gut. Nature. 2016 Dec 8;540(7632):280-283.

Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PMID: 23474465.