Composite

Part:BBa_K3606068:Design

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 09:54, 25 October 2020 by Cracra (Talk | contribs)


P12 driven mcbABCD and PtetR driven mcbEFG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4794
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4794
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4794
    Illegal BglII site found at 2204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4794
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4794
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
    Illegal NgoMIV site found at 1959
    Illegal AgeI site found at 2131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5055


Design Notes

McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.


Source

McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.

References