Coding

Part:BBa_K3468072:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 09:45, 25 October 2020 by YuanWang (Talk | contribs)


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Applications of BBa_K3468072

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase.

Fig.1 S188N in PyMOL


User Reviews

UNIQ36fa73d9549b59a5-partinfo-00000000-QINU UNIQ36fa73d9549b59a5-partinfo-00000001-QINU