Coding

Part:BBa_K3468070:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 09:41, 25 October 2020 by YuanWang (Talk | contribs)


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Please enter how you used this part and how it worked out.

Applications of BBa_K3468070

The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.

File:T260R.png
Fig.1 Thr266 in PyMOL

User Reviews

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