Coding

Part:BBa_K3468046:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:35, 25 October 2020 by YuanWang (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468046

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 143-SER. after it was mutated to ARG, it formed hydrogen bond with 138-ASN, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase.

File:Y146R.png
Fig.1 Y146R in PyMOL

User Reviews

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