Coding

Part:BBa_K3468045:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:33, 25 October 2020 by YuanWang (Talk | contribs)


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Please enter how you used this part and how it worked out.

Applications of BBa_K3468045

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 51-THR. after mutation to ARG, it formed hydrogen bond with 73-ASN and 148-LYS, which stabilized α2-β3loop and α3-β5loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 T72R in PyMOL

User Reviews

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