Coding

Part:BBa_K3468041:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:20, 25 October 2020 by YuanWang (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468041

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 169-SER and 176-LEU. after mutation to ARG, he formed new hydrogen bonds with 172-ASN, which stabilized α4-β6loop and was beneficial to improve the thermal stability of PETase.

Fig.1 N173R in PyMOL

User Reviews

UNIQ5ce09da0b683eb0f-partinfo-00000000-QINU UNIQ5ce09da0b683eb0f-partinfo-00000001-QINU