Coding

Part:BBa_K3468040:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:17, 25 October 2020 by YuanWang (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468040

It was predicted by FoldX that DDG decreased. PyOML was used for visual screening, which formed hydrogen bonds with 78-VAL, 144-Pro and 145-ILE. after mutation to ARG, it formed new hydrogen bonds with 71-PRO and 72-THR, which stabilized α3-β5loop, β2-β3loop and β2sheet, 

which was beneficial to improve the thermal stability of PETase
Fig.1 K148R in PyMOL

User Reviews

UNIQ90802e8b32f89158-partinfo-00000000-QINU UNIQ90802e8b32f89158-partinfo-00000001-QINU