Coding

Part:BBa_K3468073:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 08:56, 25 October 2020 by YuanWang (Talk | contribs)


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Applications of BBa_K3468073

It was predicted by FoldX that DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into ASP, it formed hydrogen bonds with SER-46, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.

Fig.1 Ala47 in PyMOL
Fig.2 A47D in PyMOL

User Reviews

UNIQc5cc2b36c1922419-partinfo-00000000-QINU UNIQc5cc2b36c1922419-partinfo-00000001-QINU