![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3606031:Design
Designed by: Zhenru Zhang,Yiyang Lu Group: iGEM20_Fudan (2020-10-23)
Revision as of 08:37, 25 October 2020 by Zhenru-Zhang (Talk | contribs)
Nsp4-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 135 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part Nsp4_CaAP is a combination of signal peptide Nsp4 and CaAP(Calcium Absorption Peptide). The sequence of Nsp4 is obtained from BBa_K3021004 with no further codon optimization. Then we fused Nsp4(BBa_K3606042) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.
Source
The sequences of subparts are obtained from E.coli. genome.