Regulatory

Part:BBa_K3552014

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)
Revision as of 08:31, 25 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ptac-new and lac repressor

Our new ptac promoter consists of 6 components, the reverse lac terminator, lacI, RBS, lac promoter and normal ptac promoter and lacO optimized. The new promoter comparing to the BBa_K180000 found in 2009 has a larger gradient of changing when comparing to the concentration of IPTG. In our experiment, we add different concentration of IPTG to constant promoter and received the value of florescence. We compared the value to the negative control and acquire the stable points to compare the data from the old ptac promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None