Composite
Part:BBa_K3606031:Design
Designed by: Zhenru Zhang,Yiyang Lu Group: iGEM20_Fudan (2020-10-23)
Revision as of 08:12, 25 October 2020 by Zhenru-Zhang (Talk | contribs)
Nsp4-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 135 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Part BBa_K3606031 is a new composite part designed to allow CAAP secretion from our engineered E.coli strain by adding a signal peptide novel signal peptide 4 (Nsp4) to the N terminus of CAAP. As a functional peptide that allows surface anchoring of its fused gene product, Nsp4 enables CAAP to be presented on the cellular surface of E.coli.
Source
The sequences of subparts are obtained from E.coli. genome.