Composite

Part:BBa_K3370601:Design

Designed by: CHIH-LU CHIANG   Group: iGEM20_NCTU_Formosa   (2020-10-25)
Revision as of 08:07, 25 October 2020 by Coursedjustice (Talk | contribs) (→‎Design Notes)


T7 promoter + LacO + RBS + Harmonized GR with linker and GFP + 6x His-tag + Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 689
    Illegal NgoMIV site found at 1764
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1651


Design Notes

Source

123

References