Part:BBa_K3440021

MtrB under oscillatory circuit

Plux(BBa_R0062)-RBS(BBa_B0034)-MtrB(BBa_K3146001)-DT(BBa_B0015)-Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-DT(BBa_B0015)-Prhl(BBa_R0071)-RBS(BBa_B0034)-RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)

Usage and Biology

Due to the leakiness of Prhl, RhlI is expressed and produces C4-HSL. Parallely, RhlR is constitutively expressed. The complex RhlR:C4-HSL induces Prhl. AiiA under Prhl degrades C4-HSL. Thus, with no AHL input, the circuit oscillates.

With a 3OC6-HSL input (produced by LuxI and used as quorum sensing molecule), this part can be used to produce electricity in an oscillatory manner thanks to expression of Mtrb. Indeed, LuxR is also expressed under Prhl and the complex 3OC6-HSL:LuxR activates Plux, the promoter under which Mtrb is placed.

Theoretical expectations

Improved inducible oscillatory circuit at different induction points of 3OC6-HSL. Concentrations of RhlI (I), AiiA (A), MtrB (X), C4-HSL (H), 3OC6-HSL (H2) and LuxR (R) plotted over time

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2324
  Illegal NheI site found at 2347
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4050
  Illegal BamHI site found at 2618
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4181
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3093