Signalling

Part:BBa_K3365007:Design

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 07:26, 25 October 2020 by Little ghost (Talk | contribs) (→‎Source)


Lure1 sequence downstream of pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

No.


Source

The Lure1 sequence is from the literature and ligated to the inducible pBAD/araC promoter by PCR.

References