Coding

Part:BBa_K3468088:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 07:23, 25 October 2020 by YuanWang (Talk | contribs)


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Applications of BBa_K3468088

We builded a model of the mutant (A202C-E231c), using Pymol for visual inspection (geometric criteria, see Figure 4 and Table 1), and finally using FoldX for evaluation. During visual inspection in Pymol, we found that this disulfide bond is far from the catalytic triad, far from the inside of the protein, and met the disulfide bond geometry criteria. DDG is -2.454kcal/mol evaluated by FoldX.

Fig.2 Overview of A202C and E231C

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