Regulatory

Part:BBa_K3606008

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-11)
Revision as of 05:08, 25 October 2020 by Gao (Talk | contribs)


P1 promoter

This promoter comes from a kit which contains combinations of specific constitutive bacterial promoters that vary in strength.

Design:

We have chosen a series of constitutive promoters with different strength which include P1. They are used to reliably drive expression of CaAP and McbABCEFG in E. coli in a controlled, reliable manner. We have tested these different, unique combinations to find the best expression level of CaAP and McbABCEFG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None