Part:BBa_K3596055
PpdD Signal+Pilin gene
BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. This part codes for the monomer of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires(e-PNs) along with the E.coli PpdD signal sequence to facilitate the construction of e-PNs at N terminal.
Characterization
The e-PN can be successfully expressed by express this part under the control of pTac with typeIV pilin assembly system, and the result was verified by Western Blot(figure 1).
Figure 1: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.
We made a protein film following the instructions from the paper we researched.[1] And we eventually succeeded in producing stable voltage at about 0.35V with a current of 500nA or so.(figure 2).
Figure 2: The voltage and current of e-PN
References
[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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