Coding

Part:BBa_K117000:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-07)
Revision as of 16:25, 29 October 2008 by Dariusaw (Talk | contribs) (→‎Source of Orgin sequence)

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Lysis gene (promotes lysis in colicin-producing bacteria strain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]

Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Source of Orgin sequence

to be updated

Source

to be updated

References

Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100

Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 "