Part:BBa_K3440006

RhlI-Myc under constitutive promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlI(BBa_C0070)-Myc(BBa_K823036)

Usage and Biology

Expresses RhlI under constitutive promoter. This produces C4-HSL.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Colony PCR gel for BBa_K3440007(G) and BBa_K3440008(J)

Western blot with G7 as BBa_K3440007

Coculture of chromobacterium + BBa_K3440007(G)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 722
  Illegal BglII site found at 760
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]