Composite

Part:BBa_K3440009

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 23:44, 24 October 2020 by JuCo (Talk | contribs) (→‎Characterization)


bphR2-Myc under Pconst

Pconst(BBa_J23100)-RBS(BBa_B0034)-bphR2 mutated (BBa_K1413021)-Myc (BBa_K823036)-DT(BBa_B0015)

Usage and Biology

Expresses bphR2 under a constitutive promoter

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Colony PCR gel for BBa_K3440010(M) and BBa_K3440009(L)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
origin Pseudomonas oleovorans
proteinbphr2
tagmyc