Plasmid

Part:BBa_K3582500

Designed by: Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-24)
Revision as of 16:45, 24 October 2020 by Registry (Talk | contribs)

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Modified Pet28a(+) expression vector

pET28a(+) is a novel expression plasmid generally used in Ecoli strains for protein production purposes. It has a kanamycin resistance gene as a selection marker. Plasmid sequence consists of the T7 promoter and terminator sequences as well as lac operon sequence and lac promoter for protein expression induction. Apart from this, it also has well-defined operator, promoter, MCS and tag sequences precisely inbuilt.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 1725
    Illegal NgoMIV site found at 1885
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2805


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Categories
Parameters
None