Plasmid
Part:BBa_K3582500
Designed by: Avadhoot Jadhav Group: iGEM20_IISER-Pune-India (2020-10-24)
Modified Pet28a(+) expression vector
pET28a(+) is a novel expression plasmid generally used in Ecoli strains for protein production purposes. It has a kanamycin resistance gene as a selection marker. Plasmid sequence consists of the T7 promoter and terminator sequences as well as lac operon sequence and lac promoter for protein expression induction. Apart from this, it also has well-defined operator, promoter, MCS and tag sequences precisely inbuilt.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 1725
Illegal NgoMIV site found at 1885
Illegal NgoMIV site found at 4932 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2805
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Categories
Parameters
None |