Composite

Part:BBa_K3440002

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 16:30, 24 October 2020 by JuCo (Talk | contribs) (→‎Usage and Biology)


mCherry under prma promoter

Pprma (BBa_K2911000)-RBS(BBa_B0034)-mCherry (BBa_J06504)-DT(BBa_B0015)

Usage and Biology

Produces mCherry under prma promoter, which can be activated by PFAS/PFOA

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//chassis/prokaryote/ecoli
Parameters
colorred
emission615
excitation560
originprma promoter from Rhodococcus jostii RHA1 and GFP from Anaplasma marginale