Regulatory

Part:BBa_K3402043

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-22)
Revision as of 15:27, 24 October 2020 by ZZ 0991 (Talk | contribs)

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Pugta1

Pugta1 is the promoter of UDP-glucosyltransferase A from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27
    Illegal BsaI site found at 524
    Illegal SapI.rc site found at 922


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Parameters
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