Composite

Part:BBa_K3440021

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 14:45, 24 October 2020 by JuCo (Talk | contribs)


MtrB under oscillatory circuit

Plux(BBa_R0062)-RBS(BBa_B0034)-MtrB(BBa_K3146001)-DT(BBa_B0015)-Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-DT(BBa_B0015)-Prhl(BBa_R0071)-RBS(BBa_B0034)-RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2324
    Illegal NheI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4050
    Illegal BamHI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3093


[edit]
Categories
//classic/signalling/receiver
//function/biosynthesis/ahl
//function/degradation/ahl
//function/reporter
Parameters
originShewanella oneidensis
outputOscillating electrical signal
signalling_moleculeQS of Lux system and Rhl system