Part:BBa_K3468016:Design

PETase Q127L

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around M128, L131, and V156 for mutations, the hydrophobic interaction between the residues was enhanced and the hydrophobic core was created, so Q127L was designed.

Source

Ideonalla sakaiensis