Plasmid
Part:BBa_K3505011
Designed by: Venetios Michelioudakis Group: iGEM20_Thessaly (2020-10-20)
pDGB1 omega 2 (Ω2) for Bacterial GoldenBraid
Level Ω vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Spectinomycin and ori pBBR1 for multiple protein expression capacities.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3277
Illegal PstI site found at 3316 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3277
Illegal BamHI site found at 3298 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316
Illegal NgoMIV site found at 948
Illegal NgoMIV site found at 1350 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2918
Illegal BsaI.rc site found at 6
[edit]
Categories
Parameters
//awards/part_collection
//plasmid/construction
//plasmid/expression
//plasmid/construction
//plasmid/expression
None |