![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3468086:Design
Designed by: Yufei Zhou Group: iGEM20_TJUSLS_China (2020-10-20)
PETase S58P
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 75
Design Notes
Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro, and Ser58 is at the i+1 position of β-turn. The introduction of proline mutations can theoretically improve thermal stability.
Source
Ideonalla sakaiensis