Coding

Part:BBa_K3468079:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 11:12, 24 October 2020 by YuanWang (Talk | contribs)

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PETase T113P


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro. The introduction of proline mutations can theoretically improve thermal stability.


Source

Ideonalla sakaiensis

References