Coding
Part:BBa_K3468079:Design
Designed by: Yufei Zhou Group: iGEM20_TJUSLS_China (2020-10-20)
PETase T113P
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 240
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 240
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 240
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 240
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 240
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro. The introduction of proline mutations can theoretically improve thermal stability.
Source
Ideonalla sakaiensis