Composite

Part:BBa_K3519009

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 11:02, 24 October 2020 by Omkarmohapatra123 (Talk | contribs)


medium promoter-traT

This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs. The data provided below are from previous overexpression studies in the literature.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 703
    Illegal BsaI.rc site found at 185


[edit]
Categories
Parameters
None