Composite
Part:BBa_K3431015
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-07-23)
T7 Promoter + RBS + Thermotoga maritima Invertase + T7 Terminator
This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.
Experiment result
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1356
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1127
Illegal BamHI site found at 1257
Illegal XhoI site found at 1328 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 928
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 459
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Categories
Parameters
None |