Part:BBa_K079041
Lambda OR1 - operator library member
This parts have been extracted from the Lambda Operator Library.
Lambda Fage Genetic switch is a complex and deeply characterized system. A Review on the natural systems behavior
K079041: Lambda OR1 is the most affine wild type operator sequence in the Lambda system. In the natural switch overlaps with Cro Promoter PR.
K079042: Lambda OR2 is located upstream of OR1 partially overlapping the PR promoter. cI binding affinity for the operator sequence is significantly lower than OR1 (Higher Free Energy)
K079043: Lambda OR3 recruits cI with interaction energy comparable to OR2. The different biological role in the switch systems is related to positional effects being OR3 farther from PR sequence and overlapping cI promoter P.
The resuming table clearly shows as, to determine the actual repression strength both binding affinity and [http://2008.igem.org/Team:Bologna/Modeling Positional Effects] are key elements. For Example OR3 has the strongest repression despite the lower binding affinity and a very different repression efficiency compared to the similarly binding OR2.
OR2 and OR3 have been selected despite the similar cI binding properties because OR sites can be specifically recognized as well by Cro repressor protein. In Cro interaction the increasing affinity order become OR3, OR1 and OR2, determining a very significant difference in OR2 and OR3 regulatory behavior.
Cooperative effects between naturally spaced operator sequences has been well characterized costituting a solid guideline to individual or cooperative assembly with Constitutive Promoter Family
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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