Coding

Part:BBa_K3352001:Design

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-18)
Revision as of 06:37, 24 October 2020 by Joyceting (Talk | contribs)


Φ29 DNA Polymerase with His-Tag and GS linker Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 595
    Illegal SapI.rc site found at 993


Design Notes

We obtained the amino acid sequence of Φ29 DNA polymerase from Bacillus phage Φ29. We optimized the DNA sequence for expression in E. coli and removed the EcoRI cutting site to conform to BioBrick assembly standards.


Source

We obtained the amino acid sequence of phi29 DNA polymerase from Bacillus phage phi29.

References