Composite

Part:BBa_K3490010

Designed by: Hong-Yan Huang   Group: iGEM20_NCKU_Tainan   (2020-10-24)
Revision as of 06:01, 24 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Verifying the function of tetR system.

The bacteria will continuously express TetR and GFP. After adding tetracycline into the culture, TetR will associate with tetracycline then binds on pTet which decreases the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 755
    Illegal NheI site found at 1068
    Illegal NheI site found at 1091
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None