Composite
Part:BBa_K3490010
Designed by: Hong-Yan Huang Group: iGEM20_NCKU_Tainan (2020-10-24)
Verifying the function of tetR system.
The bacteria will continuously express TetR and GFP. After adding tetracycline into the culture, TetR will associate with tetracycline then binds on pTet which decreases the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 755
Illegal NheI site found at 1068
Illegal NheI site found at 1091 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |