![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3490011
Designed by: Ren Hao Tsai Group: iGEM20_NCKU_Tainan (2020-10-24)
Causing bacteria lysis when the bacteria leak out the contact lenses.
For biosafety, we use TetR-VP16(Tet-off) system to repress the expression of lysis. The tet-off system makes use of TetR protein to associate with VP16 protein first then bind with tetracycline and further bind to TetO region to repress the expression of Lysis. Once the bacteria leak out the contact lenses which will lack of tetracycline further induce bacteria to express lysis. Hence, we can prevent the bacteria from causing damage to our eyes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 755
Illegal NheI site found at 1068
Illegal NheI site found at 1091 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |