Part:BBa_I766557

pSte5 (Weak) Promoter

Low expression, constitutive promoter in yeast
Induced by α-factor-pheromone (Printen & Sprague, 1994)
Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)

Usage and Biology

The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 (Figure 1). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.

Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 93
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 93
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 93
  
• 1000
  COMPATIBLE WITH RFC[1000]

References
Chapman, S. A., & Asthagiri, A. R. (2009). Quantitative effect of scaffold abundance on signal propagation. Molecular Systems Biology, 5(1), 313. https://doi.org/10.1038/msb.2009.73
Printen, J. A., & Sprague, G. F. (1994). Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics, 138(3), 609–619.