Generator

Part:BBa_K3580102

Designed by: Yuya Otsuki   Group: iGEM20_Waseda   (2020-10-23)
Revision as of 04:19, 24 October 2020 by Kanta (Talk | contribs)


Ptrc-trGPPS-SS(Sabinene synthase)

It is a part composed of trGPPS and sabinene synthase whose expression is regulated by Ptrc, and LacI to enable IPTG inductionof expression of those enzymes.This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in a 2015 paper by Srividya, Narayanan et al. changed the limonene synthase of Ptrc-trGPPS-LS to sabinene synthase.Sabinenecan be synthesized by combining this part with an upstream pathway that can supply the products of the mevalonate pathway (IPP and DMAPP).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1980
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2366
    Illegal XhoI site found at 4022
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1980
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1980
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None