Part:BBa_J01003

OriT-R (Origin of transfer for the R-plasmid nic region)

OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.

This oriT was characterized by Heidelberg iGEM2008 team (see experience). In this characterization the conjugation rate, conjugation time, conjugation efficiency between different strains, at different times and temperatures was measured. Furthermore the influence of donor : recipient ratio was analysed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 117
  Illegal NgoMIV site found at 127
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 19