Composite

Part:BBa_K3490021:Design

Designed by: Yao Tien Cheng   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 03:45, 24 October 2020 by Registry (Talk | contribs)

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Verify the function of EL222-pBlind and FLP-FRT systems.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1573
    Illegal SpeI site found at 1891
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1573
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3035
    Illegal NheI site found at 3058
    Illegal SpeI site found at 1891
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1573
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1573
    Illegal SpeI site found at 1891
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1573
    Illegal SpeI site found at 1891
    Illegal NgoMIV site found at 142
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2882
    Illegal BsaI.rc site found at 3115


Design Notes

during the experiment process, we had difficulty constructing both plasmids. With the doubt that the EL222-FLP system might be harmful to the growth of bacteria, we changed the design of blue-sensitive protein EL222 into thermo-sensitive protein CI857 on the plasmid pCP20 provided by our PI, Prof. Ng


Source

Derived from IDT synthesis.

References