Composite

Part:BBa_K3490020:Design

Designed by: Ren Hao Tsai   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 03:20, 24 October 2020 by Registry (Talk | contribs)

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Regulating the growth rate of bacteria.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1512
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2789
    Illegal SpeI site found at 1830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1512
    Illegal BamHI site found at 2729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
    Illegal NgoMIV site found at 142
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2225
    Illegal BsaI.rc site found at 2265


Design Notes

This year, we're unable to transform EL222 into bacteria. Hence, we decide to use CI857 to replace EL222.


Source

Derived from IDT synthesis.

References