![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3490020:Design
Designed by: Ren Hao Tsai Group: iGEM20_NCKU_Tainan (2020-10-23)
Regulating the growth rate of bacteria.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1512
Illegal SpeI site found at 1830 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1512
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2789
Illegal SpeI site found at 1830 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1512
Illegal BamHI site found at 2729 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1512
Illegal SpeI site found at 1830 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1512
Illegal SpeI site found at 1830
Illegal NgoMIV site found at 142
Illegal AgeI site found at 367 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2225
Illegal BsaI.rc site found at 2265
Design Notes
This year, we're unable to transform EL222 into bacteria. Hence, we decide to use CI857 to replace EL222.
Source
Derived from IDT synthesis.