Coding

Part:BBa_K3697002

Designed by: Christopher Neimeth   Group: iGEM20_Stanford   (2020-10-22)
Revision as of 00:33, 24 October 2020 by Cneimeth (Talk | contribs)


manP expression cassette

This part is the expression cassette for expressing manP in B. subtilis. It includes the natural RBS, Promoter, and coding sequence for the manP gene found in Bacillus Subtilis 168. Because of this, this cassette can be used in B. subtilis to produce this transporter protein at normal levels.

[1] Wenzel, M. and Altenbuchner, J. (2015) Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate‐dependent phosphotransferase system. Microbiology (United Kingdom), 161(10), 1942–1949.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 722
    Illegal AgeI site found at 372
    Illegal AgeI site found at 466
    Illegal AgeI site found at 2132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 272
    Illegal SapI site found at 1294


[edit]
Categories
//dna
//function/reporter
Parameters
biology
negative_regulators