Coding

Part:BBa_K3697002:Design

Designed by: Christopher Neimeth   Group: iGEM20_Stanford   (2020-10-22)
Revision as of 00:28, 24 October 2020 by Cneimeth (Talk | contribs) (→‎Source)

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manP expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 722
    Illegal AgeI site found at 372
    Illegal AgeI site found at 466
    Illegal AgeI site found at 2132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 272
    Illegal SapI site found at 1294


Design Notes

When designing this part, we made to sure not to subdivide the expression cassette as we wanted to insure that manP was produced at as close to a natural level as possible in the strain we used.


Source

This part was derived from the genome of Bacillus Subtilis 168 and initially used in Altenbuchner, 2015 [1]

[1] Wenzel, M. and Altenbuchner, J. (2015) Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate‐dependent phosphotransferase system. Microbiology (United Kingdom), 161(10), 1942–1949.

References