Part:BBa_K3697000
mCherry BSU
This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye.
mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm.
Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light.
This protein can be used as a reporter in B. subtilis due to it's high visibility.
This part does not have a degradation tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 715 - 1000COMPATIBLE WITH RFC[1000]
| None |