Protein_Domain

Part:BBa_K3373010:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)
Revision as of 23:41, 23 October 2020 by Registry (Talk | contribs)

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C-terminal region of TEV protease optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B5 (C-terminus coding region) position by adding BbsI sites and the corresponding fusion sites on both ends. The stop codon was added after the NLS sequence.


Source

Tobacco etch virus (TEV) 12227 [NCBI]

References