Composite
Part:BBa_K3389003
Designed by: C. Curtin, B. Cordice-Little, A. Germosen Rosa, H. Sanchez, M. Hammes, A. Ibrahim, S. Walis, L. Buchanan, C. Tarlen, E. Lopez Group: iGEM20_SUNY_Oneonta (2020-10-23)
RAD27 Flappase expression construct with 10x His tag
This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 311
Illegal BglII site found at 704 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
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Categories
Parameters
None |