Composite

Part:BBa_K3389003

Designed by: C. Curtin, B. Cordice-Little, A. Germosen Rosa, H. Sanchez, M. Hammes, A. Ibrahim, S. Walis, L. Buchanan, C. Tarlen, E. Lopez   Group: iGEM20_SUNY_Oneonta   (2020-10-23)
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RAD27 Flappase expression construct with 10x His tag

This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
    Illegal BglII site found at 704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216


[edit]
Categories
Parameters
None