Part:BBa_K3360002

Thermostable beta-glucosidase

Encoding the β-glucosidase A of Thermotoga maritima, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1577
  Illegal AgeI site found at 927
  
• 1000
  COMPATIBLE WITH RFC[1000]

SZU-China 2020 TEAM

Beta-glucosidase is a cellulase enzyme, which can catalyze the reaction of geniposide with amino acids to produce gardenia blue pigment. In order to find an enzyme that has a good tolerance to the extreme industrial environment and can achieve rapid color development in laboratory conditions, we finally created an extremely heat-resistant β-glucosidase derived from Thermotoga marina.

We chose the plasmid pGEX-4T-1-H, and added a GST tag to it and optimized its gene sequence for E. coli codons. Under IPTG induction, the enzyme can be expressed efficiently.

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