Coding

Part:BBa_K3360002:Design

Designed by: Li Wenxi   Group: iGEM20_SZU-China   (2020-10-04)
Revision as of 22:04, 23 October 2020 by Hamlet (Talk | contribs) (→‎References)

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Thermostable beta-glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1577
    Illegal AgeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

Source

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

References

Zhang Hui, Qiu Riyong, Liu Mingjie, et al. Cloning and overexpression of extremely heat-resistant β-glucosidase gene and its application in the industrial production of natural blue pigment[J].Jiangsu Agricultural Sciences,2013,41(7 ):19-22.