Coding

Part:BBa_K3360004

Designed by: Li Wenxi   Group: iGEM20_SZU-China   (2020-10-15)
Revision as of 21:30, 23 October 2020 by Hamlet (Talk | contribs) (→‎SZU-China 2020 TEAM)


endo-beta-1_4-glucanase from Streptomyces

Encoding the endo-beta-1,4-glucanase of Streptomyces.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

SZU-China 2020 TEAM

The traditional fraying process is very harmful to the environment, resources and users. In essence, being old is the destruction of cellulose on the surface of jeans. We finally consider using cellulase to achieve this effect. Through cellulase washing, it can hydrolyze the cellulose and make a part of the dye fall off the fabric. Through this effect, the "worn feeling" effect that people want can be obtained.

We chose the CL34 endoglucanase from Streptomyces. We loaded CL34 into pet-28a vector as shown below. Under the induction of IPTG, CL34 will be expressed in large quantities.



[edit]
Categories
Parameters
None